Journal: Hormones and behavior
Article Title: Nesting Behavior is Associated with VIP Expression and VIP-Fos Colocalization in a Network-Wide Manner
doi: 10.1016/j.yhbeh.2014.12.010
Figure Lengend Snippet: Representative labeling for VIP mRNA (green) and Fos immunoreactivity (red) in cross-sections from a nesting male (A, H, I) and nesting female (B-G) zebra finch. DAPI nuclear stain is shown in blue. (A) Labeling in the anterior portion of the MeA, where the MeA caps the ventral amygdalofugal pathway (vaf; also known as the occipitomesencephalic tract). (B) Labeling in the mid-caudal portion of the MeA, where the MeA is situated ventromedial to fascicles of the vaf. Asterisks denote matching locations in the main panel and inset. As shown in the inset, many VIP cells in the MeA express low levels of mRNA, although numerous cells are strongly labeled, as well. Arrows highlight VIP-Fos double-labeled neurons. (C-D) Labeling in the MPO and MPA. The asterisk in panel C corresponds to the location of the asterisk in panel D. (EG) Representative labeling in the VMH (E), AH (F) and Inf (G). (H) VIP-Fos double-labeled neurons in the VMH, as indicated by arrows. (I) Labeling in the CG, putative homologue of the mammalian ventrolateral PAG. Asterisks denote matching locations in the main panel and inset. All scale bars = 100 µm except insets, which = 25 µm.
Article Snippet: Sections were mounted on subbed slides and coverslipped with ProLong Gold antifade reagent containing DAPI nuclear stain (Invitrogen) and immediately photographed.
Techniques: Labeling, Staining